Little Brother or little brother may refer to:
Relationships
Little brother, a male sibling who is younger in age - see birth order
Little brother, a boy supported by a surrogate "big brother", through an organization such as Big Brothers Big Sisters of America
Literature
Little Brother (Baillie novel), 1985, by Allan Baillie, a children's novel about life under the Khmer Rouge
Little Brother, 1996, a novel by David Mason
"Little Brother" (short story), 2001, by Walter Mosley
Little Brother (Doctorow novel), a 2008 novel by Cory Doctorow
Religion
Little Brothers of Francis, a Franciscan order in the Anglican Communion
"Little Brother", a name for Franciscan Catholic orders
Little Brothers of St. Francis, a Roman Catholic institute of religious brothers
Little Brothers of the Lamb, a branch of a Roman Catholic religious institute, shaped both by Dominican and by Franciscan spirituality
Little Brothers of the Good Shepherd, a Roman Catholic pontifical institute of religious brothers
Little Brothers of the Gospel, a Roman Catholic congregation of religious brothers inspired by the life and writings of Charles de Foucauld
Little Brothers of Jesus, a Roman Catholic religious order based on the life and teachings of Charles de Foucauld
Little Brothers of Mary, an international community of Catholic religious institute of brothers
Organizations
International Federation of Little Brothers of the Poor, an international federation of volunteer-based non-profit organizations, committed to relieving isolation and loneliness among the elderly
Little Brothers – Friends of the Elderly, initially named Little Brothers of the Poor, a network of organizations located in the United States, member of the International Federation of Little Brothers of the Poor
Ships
USS Little Brothers (SP-921), a United States Navy tug in commission from 1917 to 1919
Films and television
Little Brother (1927 film), a Soviet film directed by Grigori Kozintsev and Leonid Trauberg
Little Brother (2014 film), a Canadian film directed by Rémi St-Michel
Little Brother (2023 film), an American film directed by Sheridan O'Donnell
Little Brother (2026 film), an American comedy film directed by Matt Spicer
"Little Brother" (Adventure Time), a 2014 TV episode
Music
ARP Little Brother, a keyboardless monophonic synthesizer expander module
Little Brother Montgomery (1906–1985), blues pianist
Little Brother (group), a hip-hop group from North Carolina, USA
Albums
Little Brother (EP) (2008), by punk band Dead to Me
Songs
"Little Brother," a song from Neil Sedaka's 1974 album Sedaka's Back
"Little Brother," a song from David Knopfler's 1983 album Release
"Little Brother", a song from Grizzly Bear's 2006 album Yellow House
"Little Brother", a song from The Future Sound of London's 1994 album Lifeforms
"Little Brother", a song by Black Star for the 1999 film The Hurricane