The Last One may refer to:
Television
"The Last One", an episode of Aqua Teen Hunger Force
"Last Last One Forever and Ever", an episode of Aqua Teen Hunger Force
"The Last One Forever and Ever (For Real This Time) (We ... Mean It)", an episode of Aqua Teen Hunger Force
"The Last One" (St. Elsewhere), the 1988 final episode of St. Elsewhere
"The Last One" (Friends), the final episode of Friends
The Last One, a documentary by Neal Hutchenson featuring moonshine maker Marvin "Popcorn" Sutton
Other meanings
The Last One (software), a computer program from the early 1980s which aimed to allow non-programmers to create application software
The Last One (album), a 2024 album by Headie One
The Last One , a Vertigo mini-series by J. M. DeMatteis
"The Last One", a song by Black Veil Brides from Vale, 2017
"The Last One", a song by Cary Brothers from Who You Are, 2007
"The Last One", a song by Hieroglyphics, from 3rd Eye Vision, 1998
"The Last One", a song by Maisie Peters from The Good Witch (Deluxe), 2023