John Holt may refer to:
Politics
John Holt (publisher) (1721–1784), publisher and mayor of Williamsburg, Virginia
John Langford-Holt (1916–1993), British member of parliament
John Holt (Australian politician) (1929–2012), New South Wales politician
Sports
John Holt (American football) (1959–2013), American football cornerback
John Holt (Australian footballer) (born 1962), for North Melbourne
John Holt (basketball), American basketball player
John Holt (cricketer) (1923–1997), West Indian cricketer
John Holt (footballer, born 1956), Scottish footballer for Dundee United
John Holt (swimmer) (1922–1966), British swimmer
Johnny Holt (1865–1937), English footballer for Everton
Other
John Holt (15th-century judge) (died 1418), English judge
John Holt (English educator) (died 1504), educator of Henry VIII
John Holt (academic) (died 1631), Oxford college head
John Holt (Lord Chief Justice) (1642–1710), Lord Chief Justice of England and Wales
John Holt (composer) (1726–1753), London bell ringer
John Holt (priest) (died 1734), Anglican priest
John Holt (author) (1743–1801), English author
John Holt (businessman) (1841–1915), English shipping merchant
John Dominis Holt II (1861–1915), Hawaiian colonel and Democratic delegate
John Dominis Holt IV (1919–1993), Hawaiian writer and cultural historian
John F. Holt (1915–1996), American medical doctor
John Riley Holt (1918–2009), English experimental physicist
John Holt (American educator) (1923–1985), American homeschooling pioneer
John Holt (singer) (1947–2014), Jamaican reggae singer
John Holt (veterinarian) (1931–2013), Australian veterinarian and Olympic shooter
John Holt & Co (Liverpool), a United Kingdom company
John Holt plc, Nigerian conglomerate